Lipase Immobilized on MCFs as Biocatalysts for Kinetic and Dynamic Kinetic Resolution of sec-Alcohols

Dynamic kinetic resolution (DKR) is one of the most attractive methods for enantioselective synthesis. In reported studies, lipase B from Candida antarctica (CALB) immobilized on siliceous mesoporous cellular foams (MCF) functionalized with different hydrophobic groups, and two ruthenium complexes substituted cyclopentadienyl ligands were investigated as catalysts chemoenzymatic DKR (rac)-1-phenylethanol, using Novozym 435 a benchmark biocatalyst. Studies (rac)-1-phenylethanol transesterification reaction showed that CALB supported MCFs grafted methyl groups promising biocatalyst isopropenyl acetate preferable acylation agent. Both Ru-complexes activated by K3PO4 or t-BuOK, proved to be effective racemization reaction. The final experiments all combinations singled out, gave 96% conversion, (R)-1-phenylethyl enantiomeric excess 98% in 8 h activator.